2nd Congress of the Spanish Network of Advanced Optica Microscopy (Program_pdf)

October 13th 2014

9:00-10:00 Registration
10:00-10:30 Welcome
10:30-11:15 Lecture 1: Plasticity of cancer cell invasion, metastasis and therapy response in vivo

Peter Friedl

11:15-11:30 Questions
11:30-12:15 Lecture 2: Fluorescence lifetime imaging across the scales- from microscopy and high content analysis to preclinical and clinical imaging

Paul French

12:15-12:30 Questions
12:30-13:00 Exhibitor presentations (Izasa & PerkinElmer)

– Point Scanning Confocal Microscopy is dead (Lachica, Edward. Andor)

– Meeting the Challenges of Physiologically Relevant Cell Model Systems. The Opera Phenix High Content Screening System (PerkinElmer)

13:00-13:15 Short-communication 1: Multimodal Nonlinear Optics Microscopy Platform Integration to Understand Cancer Transformation

Javier Adur

13:15-13:20 Questions
13:20-14:30 Lunch
14:30-15:45 Workshop 1: Open source software and macros (Auditorium)- iMSRC: intelligent screening platform: Real example of iMSRC intelligent screening applied to high resolution tissue microarray capture (Joaquim Soriano Felipe)

– Suites of plugins to enhance the capabilities of ImageJ  (Manel Bosch)

– MicroManager – How to configure and use it. Some examples (Miguel Galarraga)

Chairman: Miguel Galarraga

Collaboration: OMERO

– The Open Microscopy Environment: Open Image Informatics for the Biological Sciences (Petr Walczysko)

14:30-15:45 Workshop 2: Fluorescent markers and nanoparticles (Workshop room I. 2nd floor)

– GAP, a new aequorin-based fluorescent protein probe for imaging Ca2+ in organelles (Javier García-Sancho)

– Use of nanoparticles for the in vivo tracking of neural precursor migration in response to brain damage (Augusto Silva)

– New nanoprobes for intracelullar thermal sensing (Daniel Jaque)

Chairman: Lucía Sánchez

15:45-17:00 Workshop 3: Equipment maintenance tolos.(Workshop room I. 2nd floor)

– Tests and tools to monitor confocal microscopes’ performance (Manel Bosch)

– Tools and strategies for advanced fluorescence microscopy (Jordi Andilla)

– Performance check and optimization in widefield microscopy (Francisco Porto)

Chairman: Manuel Pérez

15:45-17:00 Workshop 4: FRAP, Photoactivation, Photoconversion. Use of Fluorescent proteins (Auditorium)

– Quantitative FRAP Analysis (José Rino)

Fluorescence Recovery After Photobleaching (FRAP) in the study of protein kinetics: data analysis, modeling and parameter inference (Nickolaos Nikiforos Giakoumakis & Maria Anna Rapsomaniki)

Chairman: María Calvo

Sponsor: Olympus

17:00-17:30 Coffe break (Delta Optics + OMERO)
17:30-19:30 REMOA meeting


October 14th 2014

9:00-9:45 Lecture 3: Quantification of intracellular intermolecular interactions by FRET/FLIM microscopy

José Requejo-Isidro

9:45-10:00 Questions
10:00-10:45 Lecture 4: Fast volumetric light-sheet microscopy

Pablo Loza

10:45-11:00 Questions
11:00-12:00 Poster session + Exhibitors

Coffe break (Zeiss)

12:00-12:45 Lecture 5: Nanoscopy 2.0

Alberto Diaspro

12:45-12:50 Questions
12:50-13:05 Short-communication 2: To What Extent Is Your Tissue Clear? Optical clearing of the mouse brain and light attenuation quantitation

Angela d’Esposito

13:05-13:10 Questions
13:10-13:25 Short-communication 3: Identification of pollen taxon from microscopic image analysis

Gabriel Cristóbal

13:25-13:30 Questions
13:30-14:30 Lunch
14:30-15:30 Exhibitor presentations (Zeiss, Hamamatsu, Izasa & Delta Optics)

– Light Sheet Z1, A New Way to View Living Things (Jacques Paysan. Carl Zeiss Microscopy)

– sCMOS cameras vs EMCCD and CCD (David Castrillo. Hamamatsu)

– New Objective Lenses for Advanced Microscopy (Luis Martín. Izasa)

– The Light Sheet Microscope for all Clearing Solutions and Water (Uwe Schröer. LaVision BioTec)

15:30-16:45 Workshop 5: Machine Learning, Supervised Image Analysis. Teach the computer to classify with no math, no parameter, no rule. (Auditorium)

– Sébastien Tosi & Julien Colombelli

Chairman: Julien Colombelli

Sponsor: Bitplane

– Imaris: Exploring the next dimension in 3D and 4D image analysis (Delisa Ibanez Garcia. Bitplane)

15:30-16:45 Workshop 6: Illumination sources and detection systems. (Workshop room I. 2nd floor)

– Which is the best scientific camera for my application? (Jorge Ripoll)

– Recent advances in microscopy illumination systems (Daniel Jaque)

– Optical Detectors for Microscopy and their Working Principles (Jordi Sobrino)

Chairman: Jorge Ripoll

Sponsor: MTB

– Two photon microscopy: Increasing imaging times using GHz laser excitation (Andy Wells. Laser Quantum)

16:45-17:15 Coffe break (Perkin Elmer)
17:15-18:30 Workshop 7: New developments and alternatives in Microscopy (Auditorium)

– Deep and clear optical imaging by using adaptive optic devices (Corinne Lorenzo)

– Compressive sensing in microscopy (Jesús Lancis)

– Raman microspectroscopy for biochemical and biomedical applications (Mónica Marro)

Chairman: Jordi Andilla

17:15-18:30 Workshop 8: FRET/FLIM and other related techniques. (Workshop room I. 2nd floor)

– Analysis of spatiotemporal signalling dynamics by FRET biosensors (Rafael Fritz)

– Design of a FRET-based biosensor to monitor phosphatidic acid fluctuations in living cells (Javier Díez Guerra)

Chairman: Timo Zimmermann

21:00 Dinner


October 15th 2014

9:00-9:45 Lecture 6: Superresolution in a Facility

Nuno Moreno

9:45-10:00 Questions
10:00-10:45 Lecture 7: Fluorescence labelling for super-resolution microscopy

Cristina Flors

10:45-11:00 Questions
11:00-11:15 Short communication 4: Biological applications for 3D STED microscope systems with two depletion lasers

Timo Zimermann

11:15-11:20 Questions
11:20-11:50 Exhibitor presentations (Leica & Vertex)

– 10 Years of Leica Super-Resolution (Juan Luis Monteagudo. Leica)

– Phenotyping TILs in situ: Automated Enumeration of FOXP3+ and CD69+ T Cells in Follicular Lymphoma (Roslyn Lloyd. PerkinElmer)

11:50-13:00 Poster session + Exhibitors

Coffe break (Leica)

13:00-13:25 Lecture 8: Super-resolution microscopy with Conical Diffraction

Spencer Shorte

13:25-13:30 Questions
13:30-14:30 Lunch
                                        FACILITY MANAGEMENT
14:30-14:50 – Addressing the Challenges of Operating a Core Facility (Lindsey Ward. iLab Solutions)

– Managing a Microscopy core with PPMS: More time to focus on Science (Jean-Yves Tinevez. Stratocore)

14:50-16:00 Core Management – An Entrepreneurial Endeavor

Tim Bushnell

16:00-17:00 RoundTable

Chairmen: Diego Megías, Carlos Sánchez & María Calvo

17:00-17:30 Final session/Farewell drinks




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